Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mertlikova-Kaiserova, H.; Rumlova, M.; Tloustova, E.; Prochazkova, E.; Holy, A.; Votruba, I.
    Point mutations in human guanylate kinase account for acquired resistance to anticancer nucleotide analogue PMEG (2011), Biochem. Pharmacol., 82, 131-138.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.8
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.8 S35N/V168F the mutations significantly suppress enzyme catalytic activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.8 0.015
-
 GMP wild type enzyme, in 100 mM Tris–HCl buffer pH 7.5, 4 mM ATP, 10 mM MgCl2, 1 mM dithiothreitol, 100 mM KCl, at 30°C Homo sapiens
2.7.4.8 0.27
-
 GMP mutant S35N/V168F, in 100 mM Tris–HCl buffer pH 7.5, 4 mM ATP, 10 mM MgCl2, 1 mM dithiothreitol, 100 mM KCl, at 30°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.8 22000
-
 x * 22000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.8 CCRF-CEM cell
-
 Homo sapiens
-
2.7.4.8 T-lymphoblast
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 ATP + GMP
-
 Homo sapiens ADP + GDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.8 ? x * 22000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.4.8 GUK
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
2.7.4.8 Homo sapiens there is an approximately 40% drop in guanylate kinase mRNA expression in cell lines resistant to 9-[2-(phosphonomethoxyethyl)guanine] and 9-[2-(phosphonomethoxyethyl)diaminopurine] down

General Information

EC Number General Information Comment Organism
2.7.4.8 physiological function guanylate kinase is the sole molecular target for the development of acquired resistance to the cytotoxic nucleotide 9-[2-(phosphonomethoxyethyl)]-guanine Homo sapiens